1.
Chinese Journal of Dermatology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-517379
ABSTRACT
Objective To establish a polymerase chain reaction(PCR) method to amplify DNA of M.leprae from fixed and paraffin- embedded tissue(FPET). Methods The DNA of M.leprae was released from FPET by using Texpat Kit and purified with 100% alcohol. The primers RPOT(1) and RPUT(2) were used to conduct the PCR. Results A total of 32 samples were examined. Out of 32 samples with BI of more than 1+ , 28 were positive for PCR. The PCR was negative in a sample with BI=0. The sensitivity of PCR reached a level of 0.04 pg DNA. Conclusion This PCR method is very useful for amplifying the DNA of M.leprae from FPET.